Our lipid detecting tool kit comprosis of 19 basic parts (11 ligand binding domains, 3 DNA binding domains and 5 other basic parts),9 composite chimeric receptors and 3 plasmid expression vectors and associated intermediates. Amongst our kit, parts of various speicies of the animalia kingdom may be found. The composite receptors are proof of concept that the basic parts acctually work. The bulk of the data (sequance, sub domains, 3d structure, ligand affinity and more..) can be found in the parts registry.
Ligand binding domains - Basic Parts
The ecdysone receptor is a nuclear receptor found in arthropods, where it controls development and contributes to other processes such as reproduction. The receptor is a non-covalent heterodimer of two proteins, the EcR protein and ultraspiracle protein (USP). It binds to and is activated by ecdysteroids. Pulses of 20-hydroxyecdysone occur during insect development, whereupon this hormone binds to the ecdysone receptor, a ligand-activated transcription factor found in the nuclei of insect cells. This in turn leads to the activation of many other genes, which ultimately causes physiological changes that result in ecdysis (moulting). Go to the official parts registry page for this part
The nuclear hormone receptor DAF-12 from Caenorhabditis elegans is activated by dafachronic acids, which derive from sterols upon oxidation by DAF-9, a cytochrome P450. DAF-12 activation is a critical checkpoint in C. elegans for acquisition of reproductive competence and for entry into adulthood rather than dauer diapause.
Go to the official parts registry page for this part
Go to the official parts registry page for this part
Go to the official parts registry page for this part
Go to the official parts registry page for this part
Go to the official parts registry page for this part
Go to the official parts registry page for this part
Go to the official parts registry page for this part
Go to the official parts registry page for this part
Go to the official parts registry page for this part
Go to the official parts registry page for this part
DNA binding domains - Basic Parts
Other Basic Parts
Chimeric Nuclear Receptors - Composite Parts
Eukaryotic expression vectors - Composite Parts
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