Team:Debrecen-Hungary/parts

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Our parts

Our lipid detecting tool kit comprosis of 19 basic parts (11 ligand binding domains, 3 DNA binding domains and 5 other basic parts),9 composite chimeric receptors and 3 plasmid expression vectors and associated intermediates. Amongst our kit, parts of various speicies of the animalia kingdom may be found. The composite receptors are proof of concept that the basic parts acctually work. The bulk of the data (sequance, sub domains, 3d structure, ligand affinity and more..) can be found in the parts registry.

Ligand binding domains - Basic Parts

Ecdysone Receptor Ligand Binding Domain (D. Melanogaster)

DAF 12 Nuclear Receptor(C. Elegans)

Nuclear hormone receptor 8 (C. Elegans)

Nuclear hormone receptor 23 (C. Elegans)

Nuclear hormone receptor 25 (C. Elegans)

Nuclear hormone receptor 31 (C. Elegans)

Nuclear hormone receptor 64(C. Elegans)

Nuclear hormone receptor 80 (C. Elegans)

Constitutive Androstane Receptor Ligand Binding Domain (H. Sapiens)

Pregnane X receptor Ligand Binding Domain (H. Sapiens)

Estrogen Receptor Ligand Binding Domain (H. Sapiens)

DNA binding domains - Basic Parts

Gal 4 DNA binding Domain (S. cerevisiae

Retinoid X Receptor DNA binding Domain (H.Sapiens)

Estrogen Receptor DNA Binding Domain (H.Sapiens)

Other Basic Parts

Vitamin D3 Receptor hinge region (H. Sapiens)

Tetracyclin responsive DNA element in a CMV protmoter (TRE-CMV)

Poly A tail type terminator

BCL-2 Associated X protein (H. Sapiens)

Chimeric Nuclear Receptors - Composite Parts

Gal4-DAF12 Chimeric nuclear receptor

Gal4-NHR8 Chimeric nuclear receptor

Gal4-NHR23 Chimeric nuclear receptor

Gal4-NHR31 Chimeric nuclear receptor

Gal4-NHR80 Chimeric nuclear receptor

Gal4-PXR Chimeric nuclear receptor

Gal4-EcR Chimeric nuclear receptor

Gal4-ER Chimeric nuclear receptor

ER DBD-VDR hinge-ER LBD Chimeric nuclear receptor

Eukaryotic expression vectors - Composite Parts

TRE-PolyA (intermediate part of the expression vector)

TRE-Gal4-PXR-PolyA in PSB1A3 (expression vector)

TRE-Gal4-EcR-PolyA in PSB1A3 (expression vector)