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Team:Calgary/7 July 2010
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'''Wednesday July 7, 2010''' | '''Wednesday July 7, 2010''' | ||
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| + | '''Raida:''' | ||
| + | *Today I completed the transformation of my construct (R0040-RBS-GFP-Terminator)in 6 different Ampicilin Resistant plates. | ||
| + | |||
| + | '''Procedure: Transformation''' | ||
| + | *1. Thaw Competent Cells | ||
| + | *2. Add 20 uL DNA | ||
| + | *3. Ice for 1/2 hour, Heat Shock 5 minutes at 37 degrees Celcius, Ice 5 min. | ||
| + | *4. Recover with 250 uL SOC for 30 minutes | ||
| + | *5. Spin down at 14 RPM, Concentrate to approximately 100 uL | ||
| + | *6. Plate 25 uL on plate 1,2,3 and Plate 50 uL on plate 4,5,6 | ||
| + | *7. Leave in Incubator overnight for 20 hours | ||
| + | |||
| + | ''The plates are labeled as following:'' | ||
| + | *P1: R0040-I0504-PSB1A2 (construct tube C 6 July) 25 uL | ||
| + | *P2: R0040-I0504-PSB1A2 (construct tube D 6 July) 25 uL | ||
| + | *P3: R0040-I0504-PSB1A2 (construct tube F 6 July) 25 uL | ||
| + | *P4: R0040-I0504-PSB1A2 (construct tube C 6 July) 50 uL | ||
| + | *P5: R0040-I0504-PSB1A2 (construct tube D 6 July) 50 uL | ||
| + | *P6: R0040-I0504-PSB1A2 (construct tube F 6 July) 50 uL | ||
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| + | **Then I left the plates in the Incubator which is at 37 degrees Celcius. I will leave it there until 10:00 am tomorrow (July 8th), which would let the cells grow for 20 hours. | ||
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Revision as of 18:19, 7 July 2010
Wednesday July 7, 2010
Raida:
- Today I completed the transformation of my construct (R0040-RBS-GFP-Terminator)in 6 different Ampicilin Resistant plates.
Procedure: Transformation
- 1. Thaw Competent Cells
- 2. Add 20 uL DNA
- 3. Ice for 1/2 hour, Heat Shock 5 minutes at 37 degrees Celcius, Ice 5 min.
- 4. Recover with 250 uL SOC for 30 minutes
- 5. Spin down at 14 RPM, Concentrate to approximately 100 uL
- 6. Plate 25 uL on plate 1,2,3 and Plate 50 uL on plate 4,5,6
- 7. Leave in Incubator overnight for 20 hours
The plates are labeled as following:
- P1: R0040-I0504-PSB1A2 (construct tube C 6 July) 25 uL
- P2: R0040-I0504-PSB1A2 (construct tube D 6 July) 25 uL
- P3: R0040-I0504-PSB1A2 (construct tube F 6 July) 25 uL
- P4: R0040-I0504-PSB1A2 (construct tube C 6 July) 50 uL
- P5: R0040-I0504-PSB1A2 (construct tube D 6 July) 50 uL
- P6: R0040-I0504-PSB1A2 (construct tube F 6 July) 50 uL
- Then I left the plates in the Incubator which is at 37 degrees Celcius. I will leave it there until 10:00 am tomorrow (July 8th), which would let the cells grow for 20 hours.
Placeholder Text
No notebook page exists for this date. Sorry!
