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Team:Calgary/31 August 2010
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Mostly wiki stuff today. All of the pages should be created now. Now it's a matter of adding the content to them all. I am also hoping to get the folding portion of the animation done for Maya by the weekend. I am not certain how well I will be able to meet the deadline, but we shall see. | Mostly wiki stuff today. All of the pages should be created now. Now it's a matter of adding the content to them all. I am also hoping to get the folding portion of the animation done for Maya by the weekend. I am not certain how well I will be able to meet the deadline, but we shall see. | ||
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| + | <u>Himika</u> | ||
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| + | Today I miniprepped the overnight cultures from yesterday which were of I0500-B0034-MalE31.This was done because I needed more plasmids for construction. I also constructed DegP+GFP generator and DegP+RFP generator with I0500-B0034-MalE31. I used DegP+GFP/RFP generator as vectors and the MalE31 circuits as inserts. The were plated on AK. I also induced CpxR circuit with MalE31 (C4, C6) with 0.15,0.2,0.25,0.3,0.35,0.4,0.45,0.5,0.6 percent arabinose. Tomorrow I will read the GFP output of these on a plate reader to see if the induction works! | ||
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Revision as of 23:12, 31 August 2010
Tuesday August 31, 2010
Patrick
Mostly wiki stuff today. All of the pages should be created now. Now it's a matter of adding the content to them all. I am also hoping to get the folding portion of the animation done for Maya by the weekend. I am not certain how well I will be able to meet the deadline, but we shall see.
Himika
Today I miniprepped the overnight cultures from yesterday which were of I0500-B0034-MalE31.This was done because I needed more plasmids for construction. I also constructed DegP+GFP generator and DegP+RFP generator with I0500-B0034-MalE31. I used DegP+GFP/RFP generator as vectors and the MalE31 circuits as inserts. The were plated on AK. I also induced CpxR circuit with MalE31 (C4, C6) with 0.15,0.2,0.25,0.3,0.35,0.4,0.45,0.5,0.6 percent arabinose. Tomorrow I will read the GFP output of these on a plate reader to see if the induction works!
