Team:Calgary/14 May 2010

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'''Friday May 14, 2010'''
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Friday May 14, 2010|
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Today, we observed the plates where the R0040 GFP was added into competent cells under UV light. The plates had large quantities of growth only on plates containing ampicilin. No growth was observed on plates containing kanamycin. For the rest of the day, we continued research into the topics that we had narrowed our possible projects down to.
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Today, we observed the plates from which the R0040 GFP part was added into competent cells under UV light. With that light, the Green fluorescent protein should be much more visible. The plates with ampicillin showed large quantities of growth while the plates with kanamycin had no growth whatsoever. We grew them on both plates because we were unsure which antibiotic resistance the R0040 GFP part contained. With this knowledge, we confirmed that the Competent cells as well as the GFP R0040 GFP were functional. In the future, we would confirm the part was present through PCR and subsequent agarose gel electrophoresis. For the rest of the day, we continued research into our topics that we had narrowed the project list down to.
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Latest revision as of 02:49, 23 August 2010

Friday May 14, 2010

Today, we observed the plates from which the R0040 GFP part was added into competent cells under UV light. With that light, the Green fluorescent protein should be much more visible. The plates with ampicillin showed large quantities of growth while the plates with kanamycin had no growth whatsoever. We grew them on both plates because we were unsure which antibiotic resistance the R0040 GFP part contained. With this knowledge, we confirmed that the Competent cells as well as the GFP R0040 GFP were functional. In the future, we would confirm the part was present through PCR and subsequent agarose gel electrophoresis. For the rest of the day, we continued research into our topics that we had narrowed the project list down to.