User contributions
From 2010.igem.org
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- 08:33, 23 September 2010 (diff | hist) N Team:UT-Tokyo/Agarose Gel (New page: __NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} <h1>Agarose Gel</h1> <h2>Preperation</h2> *・Agarose *・TAE buffer *・MilliQ <h1>Procedure</h1> To make 1% agarose gel, make 200 ml (3 b...) (top)
- 08:30, 23 September 2010 (diff | hist) N Team:UT-Tokyo/LB broth (New page: __NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} <h1>LB broth</h1> <h2>Preparation</h2> *・Bacto trypton *・NaCl *・Bacto Yeast *・MilliQ *・Agarose (for plate) <h2>Procedure</h2> 1...) (top)
- 08:24, 23 September 2010 (diff | hist) N Team:UT-Tokyo/Gel DNA Recovery (New page: __NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} <h1>Gel DNA Recovery</h1> <h2>Preparation</h2> *・Kit of promega *・MilliQ *・Gel *・Make sure that tank for electrophoresis and EtBr are ...) (top)
- 08:21, 23 September 2010 (diff | hist) Team:UT-Tokyo/Electrophoresis (top)
- 08:20, 23 September 2010 (diff | hist) Team:UT-Tokyo/Miniprep (top)
- 08:19, 23 September 2010 (diff | hist) Team:UT-Tokyo/Miniprep
- 08:18, 23 September 2010 (diff | hist) Team:UT-Tokyo/Miniprep
- 08:15, 23 September 2010 (diff | hist) N Team:UT-Tokyo/Electrophoresis (New page: __NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} <h1>Electrophoresis</h1> <h2>Preparation</h2> *・Sample *・Gel (1% gel for fine separation, 2% for rough separation, use 2 % gel for colon...)
- 08:12, 23 September 2010 (diff | hist) N Team:UT-Tokyo/Colony PCR(Ex-Taq) (New page: __NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} <h1>Colony PCR(Ex-Taq)</h1> <h2>Preparation</h2> *・5′primer *・3′primer *・2.5mMdNTP *・10x standard buffer *・MilliQ *・DNA temp...) (top)
- 08:05, 23 September 2010 (diff | hist) Team:UT-Tokyo/PCR(KODplus) (top)
- 08:04, 23 September 2010 (diff | hist) Team:UT-Tokyo/PCR(KODplus)
- 08:02, 23 September 2010 (diff | hist) N Team:UT-Tokyo/PCR(KODplus) (New page: __NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} <h1>PCR(KODplus)</h1> Components Volume Final Concentration 10× PCR Buffer for KOD -Plus- 5 μl 1× 2mM dNTPs 5 μl 0.2mM each 25mM MgSO4 2 μ...)
- 07:58, 23 September 2010 (diff | hist) N Team:UT-Tokyo/PCR(Ex-Taq) (New page: __NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} <h1>PCR(Ex-Taq)</h1> <h2>Preparation</h2> *5′primer *3′primer *2.5mMdNTP *10xstanderd buffer *MilliQ *template DNA *Ex-Taq <h2>Procedur...) (top)
- 07:52, 23 September 2010 (diff | hist) Team:UT-Tokyo/PCR(Pfu Ultra) (top)
- 07:50, 23 September 2010 (diff | hist) N Team:UT-Tokyo/PCR(Pfu Ultra) (New page: __NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} <h1>PCR(Pfu Ultra)</h1> <h2>Preparation</h2> *5′primer *3′primer *2.5mMdNTP *10xPfu Ultra 2 buffer *MilliQ *template DNA *Pfu Ultra2 <h2>...)
- 07:46, 23 September 2010 (diff | hist) Talk:Team:UT-Tokyo (top)
- 07:44, 23 September 2010 (diff | hist) Team:UT-Tokyo/Restriction Enzyme digestion —One by one (top)
- 07:42, 23 September 2010 (diff | hist) N Team:UT-Tokyo/Restriction Enzyme digestion —One by one (New page: __NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} <h1>Restriction Enzyme digestion —One by one</h1> <h2>Procedure(20μl)</h2> ! use 5ml tube<br /> 1. add 1ng plasmid<br /> ...)
- 07:37, 23 September 2010 (diff | hist) Team:UT-Tokyo/Restriction Enzyme digestion(Xba1/Pst1)—Once for all (top)
- 07:34, 23 September 2010 (diff | hist) N Team:UT-Tokyo/Restriction Enzyme digestion(Xba1/Pst1)—Once for all (New page: __NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} <h1>Restriction Enzyme digestion(Xba1/Pst1)—Once for all</h1> <h2>Preparation</h2> *plasmid *10× buffer (H and M in the freezer) *enz...)
- 07:28, 23 September 2010 (diff | hist) N Team:UT-Tokyo/Miniprep (New page: __NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} <h1>Miniprep</h1> <h2>Preparation</h2> *kit of Promega (SVMinipreps) *incubative tube *2ml epp tube *1.5ml epp tube *MilliQ <h2>Procedure</h...)
- 07:24, 23 September 2010 (diff | hist) Team:UT-Tokyo/Colony Pick Up (top)
- 07:21, 23 September 2010 (diff | hist) N Team:UT-Tokyo/Colony Pick Up (New page: __NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} Preparation *test tube *LBbroth 4ml/tube *antibiotics (take out from fridge and use after thawing out / needless to use after colony PCR) Pro...)
- 07:17, 23 September 2010 (diff | hist) Team:UT-Tokyo/Protocols
- 07:16, 23 September 2010 (diff | hist) Team:UT-Tokyo/Protocols
- 07:05, 23 September 2010 (diff | hist) Team:UT-Tokyo/Transformation (top)
- 07:02, 23 September 2010 (diff | hist) Team:UT-Tokyo/Transformation
- 07:00, 23 September 2010 (diff | hist) Team:UT-Tokyo/Transformation
- 06:44, 23 September 2010 (diff | hist) Team:UT-Tokyo/Protocols
- 06:40, 23 September 2010 (diff | hist) N Team:UT-Tokyo/Transformation (New page: a)
- 06:39, 23 September 2010 (diff | hist) Team:UT-Tokyo/Protocols
- 06:38, 23 September 2010 (diff | hist) Team:UT-Tokyo/Protocols
- 06:36, 23 September 2010 (diff | hist) Team:UT-Tokyo/Protocols
- 06:30, 23 September 2010 (diff | hist) Team:UT-Tokyo/Protocols
- 06:02, 23 September 2010 (diff | hist) Team:UT-Tokyo/Protocols
- 06:01, 23 September 2010 (diff | hist) Team:UT-Tokyo/Protocols
- 06:01, 23 September 2010 (diff | hist) Team:UT-Tokyo/Protocols
- 05:58, 23 September 2010 (diff | hist) Team:UT-Tokyo/Protocols
- 05:56, 23 September 2010 (diff | hist) Team:UT-Tokyo/Protocols
- 05:55, 23 September 2010 (diff | hist) N Team:UT-Tokyo/Protocols (New page: __NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} <p class="pad"> ! Notice ! -You should mix reagents well -Put in enzymes after you mixed all other reagents -Centrifuge the tube and collect s...)
- 04:54, 23 September 2010 (diff | hist) Team:UT-Tokyo/Bio result (top)
- 04:53, 23 September 2010 (diff | hist) Team:UT-Tokyo/Bio lab note (top)
- 04:53, 23 September 2010 (diff | hist) Team:UT-Tokyo/Bio construct
- 04:52, 23 September 2010 (diff | hist) Team:UT-Tokyo/Bio abstract
- 04:50, 23 September 2010 (diff | hist) Team:UT-Tokyo/Allergy lab note (top)
- 04:48, 23 September 2010 (diff | hist) Team:UT-Tokyo/Allergy construct
- 04:48, 23 September 2010 (diff | hist) Team:UT-Tokyo/Allergy construct
- 04:47, 23 September 2010 (diff | hist) Team:UT-Tokyo/Allergy abstract
- 04:46, 23 September 2010 (diff | hist) Team:UT-Tokyo/Sudoku result
- 04:44, 23 September 2010 (diff | hist) Team:UT-Tokyo/Sudoku lab note
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)