Colony Pick Up

Preparation

• test tube
• LBbroth 4ml/tube
• antibiotics

(take out from fridge and use after thawing out / needless to use after colony PCR)

Procedure

• 1. add 2 μm of LB broth to the tube
• 2. add the antibiotic to the tube(1μm for 1ml)
• 3. poke the colony on the plate by toothpick(or tip of pipette)and put the toothpick to the tube
• 4. label the cap of the tube and fix the cap on the tube
• 5. incubate it at 37 degrees (shake culture)
  

(15～18 hours is best / 8 hours is the fastest)