Team:UT-Tokyo/Electrophoresis

From 2010.igem.org

UT-Tokyo

Electrophoresis

Preparation

  • ・Sample
  • ・Gel (1% gel for fine separation, 2% for rough separation, use 2 % gel for colony PCR)
  • ・DNA loading dye
  • ・DNA λH200 ladder mix

Procedure

  • 1. Add 1 ul dye for 10 ul sample, and apply to the well.
  • 2. Apply 2 ul ladder to the edge well, electrophoresis for 30 minutes.
    • ! DNA chain fewer than 100 bp has danger of over flowing.If you do electrophoresis for 27 minutes, chain of 129 bp will narrowly stay in the gel.
  • 3. Dye with EtBr for about 20 minutes.
  • 4. Observe by UV.
    • Use “Fujifilm” to cut out. This one needs time to boot-up, so turn on the switch first.