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11 August 2010
From 2010.igem.org
(Difference between revisions)
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[Details of Experiment]<BR> | [Details of Experiment]<BR> | ||
(1)We made 1% Agarose Gel by using Agarose 0.2g and TEA 20μL.<BR> | (1)We made 1% Agarose Gel by using Agarose 0.2g and TEA 20μL.<BR> | ||
| - | (2)We mixed DNA 5μL, Marker 1μL and Loading Buffer 1μL.We applied each 5μL of them to gel.We applied Marker 5μL to the far left lane.<BR> | + | (2)We mixed DNA 5μL, Marker 1μL and Loading Buffer 1μL.<br> |
| + | We applied each 5μL of them to gel.We applied Marker 5μL to the far left lane.<BR> | ||
(3) 100V, 30min electrophoresed<BR> | (3) 100V, 30min electrophoresed<BR> | ||
(4)Gel soaked in EtBr for 20min.<BR> | (4)Gel soaked in EtBr for 20min.<BR> | ||
Revision as of 01:45, 21 August 2010
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