Team:HokkaidoU Japan/Notebook/September9
From 2010.igem.org
3 piece ligation of HSP, GFP and pSB1C3
HSP | PCRed | Gel extracted | Digestion |
GFP | Previously purified by gel extraction | - | Digestion |
pSB1C3 | Previously purified by gel extraction | - | Digestion |
- Electrophoresed HSP and the same time also did GFP electrophoresis
- GFP band was very weak
- After gel extraction electrophoresed both both HSP and GFP
- Now both bands were too weak
- Retry PCR next day