Team:Alberta/Notebook/protocols/pcr purification

TEAM ALBERTA

• HOME
• AT A GLANCE
• PROJECT
  • Overview

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  • BioBytes 2.0

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  • Software

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  • Modelling

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  • The Kit

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  • Kit Components
• ACHIEVEMENTS
  • Overview

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  • Medal Requirements

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  • Media
• HUMAN PRACTICES
  • Overview

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  • Distribution Analysis

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  • High School

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  • DiscoverElle Visit

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  • Safety
• PARTS
• TIMELINE & PROTOCOLS
  • Overview

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  • General Protocols

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  • Reusable Plates

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  • Base Plasmids

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  • Creating Parts

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  • Transforming Cells

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  • Anchor

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  • Beads

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  • Optimizations

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  • Software
• TEAM

General Protocols

• In Vitro BioByte Assembly

• LB Plates and Broth

• Transformations

• 5mL Overnight

• Glycerol Stock

• Plasmid Miniprep

• Restriction Digest

• Vector Dephosphorylation

• Ligation

• Agarose Gel Electrophoresis

• Gel Extraction

• PCR

• Colony PCR

• PCR Purification

• Sample Labelling Conventions

• Fluorescent Sequencing Reaction

• Primer Design