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Team:Alberta/Kit components
From 2010.igem.org
The DNA Components
Open Reading Frame Parts
| Fluorescent Proteins | Carotenoid Pathway | Violacein Pathway | Transcription Factors | Resistance Marker |
|---|---|---|---|---|
| GFP | CrtE | VioA | LacI | Ampicillin |
| mRFP | CrtB | VioB | AraC | Chloramphenicol |
| CFP | CrtI | VioD | LambdaCI | Kanamycin |
| YFP | CrtY | VioE | TetR | Tetramycin |
Linker Parts
- Transcription
- 5 Anderson Promoters
- Operon Linker
- Anti-linker as a negative control
- Short null-linker for inversion of ends
- Fusion Protein Link
- Protein Tags
Other Parts
- High, medium and low copy number Oris
- Caps
- Anchors
Other Components
These parts and a microwave or hotplate are all that is needed to start building with GENOMIKON. (All components are pre-sterilized.)
- Precision Droppers to accurately dispense small volumes of liquids
- Magnetic Racks
- Eppendorf Tubes
- Wash Buffer
- Elution Buffer
- Lyophilized Competent Cells
- LB media
- Plates
- Iron Micro Beads
- Thermometer
