Plasmid Miniprep


  • Overnight culture
  • Qiagen plasmid miniprep kit


  • Transfer 1.5ml of culture to Eppendorf.
  • Spin down at 13 000 rpm.
  • Discard supernatant and follow procedure on p. 22 of QIAprep Miniprep Handbook.
  • Determine concentration of purified plasmid with nanodrop.


  • Low speed pellet is easier to resuspend.
  • To resuspend, take an empty micro centrifuge rack and rub the tube quickly over the apparatus and vortex.
  • Step 2 in Qiagen, the alkaline lysis with buffer P2, should be brief to avoid damaging DNA.