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Contents 1 6 October 2010 Meeting 1.1 Roll call 1.2 Presentation 1.2.1 Introduction 1.2.2 Bacilla Filla slide 1.2.3 Technical review slide 1.2.4 Swarming slide 1.2.5 Subtilin slide 1.2.6 Subtilin immunity slide 1.2.7 Workflow slide 1.2.8 YneA slide 1.2.9 Glue slide 1.2.10 Kill switch 1.2.11 Ethic slide 1.2.12 Others 1.2.13 Urease slide 1.3 Lab feedback 1.3.1 Filamentous cells 1.3.2 SR1 1.3.3 Scanning electron microscopy 1.4 Agenda 1.5 Action points 1.6 Next meeting

6 October 2010 Meeting

Roll call

• Apologies: Colin Harwood, Jem and Phil

Presentation

Introduction

• Mention why do we use this bug and that it is able to live in high pH
• Have to menetion that we all biobrick have been modelled by SBML and copasi

Bacilla Filla slide

• The solution words to be smaller than the logo
• Logo have to be the biggest!
• Solution at the top of the page with a small logo below it then zoom into the logo

Technical review slide

• Show the link between the genes
• Some of the summary slide's information to go here, example: swim down the cracks, the tent, spray on, etc.
• A single shot overview then to the biobricks
• SAY: This is a crack, and here is our bug on the surface of the crack, etc
• Label the pictures!
• Animation for the overview, ie, the background to remain the same

Swarming slide

• Change the flagella of the bug
• Zoom into the plates

Subtilin slide

• Show subtilin only when the bug is at the end of the crack
• Fill up the crack with bugs
• Describle the subtilin signalling system
• Change the pveg promoter to the oxygen sensitive promoter

Subtilin immunity slide

• SAY: Subtilin immunity ablready in the registry, that is why we use it
• Mention what the individual CDS do
• Green tick for those that we did and a red cross for those that we did not

Workflow slide

• Change the word interesting to valuable to all synthetic biologist
• Cut down on the script
• Have to make it fit to the presentation
• Integration of lots of data that is why we use e-science

YneA slide

• Put filamentous cells into the pictures
• Metabolic pathway picture and link it

Glue slide

• The iGEM plates to go onto it

Kill switch

• Can bin it
• Move to the ethic page

Ethic slide

• Move it to the front after the introduction and also mention in the summary
• What is the danger and how we make it safe

Others

• Show that the bug is able to grow in concrete

Urease slide

• Have to show that calcium carbonate is filling up the cracks
• Have to say that we are better than the rest
• Highlight the advantage of ours and the disadvantage of others
• Mention SR1

Lab feedback

Filamentous cells

• Redo the microscopic
• Correlate between the expression of GFP and the length

SR1

• We have colonies

Scanning electron microscopy

• The concrete samples are already with the EM unit

Agenda

• To reedit the agenda

Action points

• Rachel and Phil: Characterisation data for filamentous cells brick needs to be put on the wiki and the parts registry.
• Everyone to do up the presentation slides first.

Next meeting

Wed 4pm.

• Chair: Steven
• Computer: Alan
• Minutes: Deena