Team:Peking

       Biosensor:
       Based on reverse engineering principles, we
   engineered our bacteria into valid bioreporters
   for Hg(II) in aquatic environment. Rational desi-
   -gn of genetic circuit topology was conducted to
   confer mercury sensor and regulator compone-
   -nts high efficiency and robustness. Additionally,
    a new approach based on the use of multiple
   bioreporter cell lines to control assay variations
   and to improve in field application ease was also
   developed.
                                           learn more

       Expansion:    MerR family TFs share a high homology at their
   metal binding domains, which implies that our strategies of bioreporter
   and bioabsorbent engineering might be applicable to other cases. Then our
    reverse engineering strategy was expanded to another common toxic heavy
    metal, lead (Pb) to prove the validness of our engineering strategy.learn more