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Contents 1 6 October 2010 Meeting 1.1 Roll call 1.2 Presentation 1.2.1 Bacilla Filla slide 1.2.2 Technical review slide 1.2.3 Swarming slide 1.2.4 Subtilin slide 1.3 Lab feedback 1.3.1 Filamentous cells 1.3.2 SR1 1.3.3 Scanning electron microscopy 1.4 Agenda 1.5 Action points 1.6 Next meeting

6 October 2010 Meeting

Roll call

• Apologies: Colin Harwood and Jem

Presentation

Bacilla Filla slide

• The solution words to be smaller than the logo
• Logo have to be the biggest!
• Solution at the top of the page with a small logo below it then zoom into the logo

Technical review slide

• Show the link between the genes
• Some of the summary slide's information to go here, example: swim down the cracks, the tent, spray on, etc.
• A single shot overview then to the biobricks
• SAY: This is a crack, and here is our bug on the surface of the crack, etc
• Label the pictures!
• Animation for the overview, ie, the background to remain the same

Swarming slide

• Change the flagella of the bug
• Zoom into the plates

Subtilin slide

• Show subtilin only when the bug is at the end of the crack
• Fill up the crack with bugs
• Describle the subtilin signalling system
• Change the pveg promoter to the oxygen sensitive promoter

Lab feedback

Filamentous cells

• Redo the microscopic
• Correlate between the expression of GFP and the length

SR1

• We have colonies

Scanning electron microscopy

• The concrete samples are already with the EM unit

Agenda

• To reedit the agenda

Action points

• Rachel and Phil: Characterisation data for filamentous cells brick needs to be put on the wiki and the parts registry.
• Everyone to do up the presentation slides first.

Next meeting

Wed 4pm.

• Chair: Steven
• Computer: Alan
• Minutes: Deena