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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/03
From 2010.igem.org
Contents |
2010/10/03
1:PCR
<Member>
hito
<Sample>(temperature in annealing:℃)
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 1-2
- 4-5
- 7-8-10
<Protocol>
See Protocol 2
2：DNA extraction
＜Member＞
mio
＜Sample＞
PCR products
- 4②
- 4⑤
＜Protocol＞
See Protocol 4
3:Electrophoresis
<Member>
nito
<Sample>
All PCR products(10/3)
<Protocol>
See Protocol 8
<Result>
4：DNA extraction
＜Member＞
mio,mizuki,nito
＜Sample＞
PCR products
- 1
- 3
- 4
- 5
- 8
- 10
- 4-5
- 7-8-10
- 2
- 6
Digestion products
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 7-8-10
＜Protocol＞
See Protocol 4
5：DNA Ligation
＜Member＞
mio,mizuki
＜Sample＞
- 1-2
- 4-5
- 6A-7
- 7-8-10
(after digestion)
＜Protocol＞
See Protocol 10
6:Transformation
<Member>
nito
<Sample>
- 7-8-10
- JM109
<Protocol>
See Protocol 7
7：DNA Digestion
＜Member＞
nito
＜Sample, Materials＞
・PCR productions
- 1
- 2
- 3
- 4
- 6
- 10
・Digest enzyme
- Avr2
- Nhe1
＜Protocol＞
See Prptpcpl 9
