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Team:SDU-Denmark/labnotes11
From 2010.igem.org
Lab notes (9/20 - 9/26)
Contents |
Photosensor group
Miniprep of PS (composit part) in pSB3T5
Date: 9/22 2010
Done By: Lc and Maria
Protocol: MP1.2
Notes:
col. N and J from the coloni PCR was selected and miniprep'ed according to protocol. Sample 1 of each colni was eluted in 20uL elution buffer, and sample 2 of each coloni was eluted according to protocol.
To ensure thet the plasimids contained the correct insert, the miniprep'ed plasmids were digested with EcoRI and PstI.
Restriction mixture:
12uL H2O
2uL FD green buffer
1uL EcoRI
1uL PstI
5uL Plasmid from either col. N or J
The samples are digested for 10 min. at 37C, and samples are loaded onto a 1% agarose gel. Undigested plasmid is used as controle and gene ruler DNA ladder mix is used as marker.
Loading scheme:
| Lanes | Sample |
| 1 | marker |
| 2 | col. J |
| 3 | col J (uncut) |
| 4 | col N |
| 5 | col N (uncut) |
Results:
Analysis:
Two bands are observed en the lanes of the digestes samples representing undigested plasmid and insert.
Miniprep samples are sent for sequencing and is stored at -20C.
--Tipi 11:58, 30 September 2010 (UTC)
Flagella Group
Restriction Digest of FlhDCmut-B0015 and pSB3T5 with J13002
Date: 9/25 2010
Done By: Louise, Pernille and Sheila
Protocol: RD1.1 and DE1.3
Notes:
Results: gel extraction
Conclusion:
Ligation of pSB3T5 with J13002 and FlhDCmut-B0015
Date: 9/25 2010
Done By: Louise, Pernille and Sheila
Protocol: LG1.2
Notes: Samples were prepared in the ratios 1:1, 1:2 and 1:4
Results:
Conclusion:
Transformation of FlhDCmut-B0015 inserted into pSB3T5 with J13002
Date: 9/26 2010
Done By: Louise and Sheila
Protocol: CC1.1 and TR1.1
Notes:
Results: Can be seen here and here.
Conclusion:
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