Lab notes (9/20 - 9/26)
Miniprep of PS (composit part) in pSB3T5
Date: 9/22 2010
Done By: Lc and Maria
col. N and J from the coloni PCR was selected and miniprep'ed according to protocol. Sample 1 of each colni was eluted in 20uL elution buffer, and sample 2 of each coloni was eluted according to protocol.
To ensure thet the plasimids contained the correct insert, the miniprep'ed plasmids were digested with EcoRI and PstI.
2uL FD green buffer
5uL Plasmid from either col. N or J
The samples are digested for 10 min. at 37C, and samples are loaded onto a 1% agarose gel. Undigested plasmid is used as controle and gene ruler DNA ladder mix is used as marker.
|3||col J (uncut)|
|5||col N (uncut)|
Two bands are observed en the lanes of the digestes samples representing undigested plasmid and insert.
Miniprep samples are sent for sequencing and is stored at -20C.
--Tipi 11:58, 30 September 2010 (UTC)
Restriction Digest of FlhDCmut-B0015 and pSB3T5 with J13002
Ligation of pSB3T5 with J13002 and FlhDCmut-B0015
Date: 9/25 2010
Done By: Louise, Pernille and Sheila
Notes: Samples were prepared in the ratios 1:1, 1:2 and 1:4
Transformation of FlhDCmut-B0015 inserted into pSB3T5 with J13002
Insert clever content here.