Team:Brown/Notebook/September17

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Friday, September 17, 2010

Transformation of pBS+TatLinker ligation (9/16) into XL1B

Started at 6:15PM:

  • Added 3ul ligation product into 150ul cell aliquot
  • Incubated 30min ice
  • Heat shocked @42C for 1min
  • Added 100ul LB
  • Plated 200ul on LB amp plate
  • Incubated @37C
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