Team:Newcastle/Meetings/15 September 2010
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Contents |
Roll calls
- Apologies: Phil and Deena
- Absence: Colin Harwood, Colin Davie, Da Ye, Wendy
- Concrete has been made, set in tubes.
- We went to the EM facility, won't be a problem to take photos of our concrete.
- Wiki design should be ready soon (Harsh's graphic designer friend).
Lab feedback
- pMutin4 strain transformation is not going very well. Don't just sit and wait to see if Bacillus transformation worked, do another transformation on the assumption that it hasn't.
- Do chromosomal preps from both strains (168 and 168 with pMutin4), transform with the chromosome from the opposite strain.
- As an alternative approach, Jem's LacI plasmid is here, details will be given on a sheet.
- SR1 transformed. Check that it is coming in pSB1C3 too.
- Hyperspank
- fluoresence reader and microscope
- Fluostar (Phil Aldridge/Richard?)
- Transformations of LacI E. coli strain today
- Hyperspankoid in pSB1C3
- Arabinose repressor in pSB1AK3. Has to go into BS.
Concrete
- Try
- Need to make the levans plates with "iGEM" letters
- Try to stick our concrete sticks together
Modelling
Flux balance analysis on wiki by next week
Action points
- Neil and Jen - To register the team.
- Alan - To email Jen about the IPTG plasmid and the letters for the sucrose plates.
- Harsh - To crack the concrete into smaller pieces.
- Wendy - To talk to the EM people.
Next meeting
- Chair: Jannetta, Minutes: Steven, Computer: Alan
- Wednesday morning, 9am, CBCB.