Team:Freiburg Bioware/NoteBook/Labjournal/March

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1. Labday 31.03.2010

Sequence analysis

Investigators: Christian W., Bea, Kira, Achim, Kerstin, Hanna, Christian L. (Tobias, Kristian)


Theoretical cloning


Sequence analysis (Alignement) of the Stratagene Vector with

  • AAV2
  • AAV5
  • AAV7
  • AAV8

result: Media:AAV_alignment.pdf
The alignment showed significant differences in the sequences of several AAV genomes, resulting in frame shifts. We decided to rely on the Stratagene sequence

To do:

  • download and compare further AAV serotypes. (In Geneious)
  • compare literature
  • find out which possibilities are given to modify the Capsid

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