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Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/29
From 2010.igem.org
Contents |
2010/08/29
1：PCR
＜Member＞
Mariko, nito
＜Sample＞
・E-coli (having BBa_I13521 plasmid)
On 8/28, we used incorrect plasmid, so we had PCR again.
＜Protocol＞
See Protocol 2
・Tube (temperature in annealing…HH/H/L/LL)
3. mRfp~terminator (69.0℃/67.5/66.0/63.0)
7. Terminator (69.0/67.5/66.0/63.0)
Total 8 tubes.
2：DNA extraction
＜Member＞
nito
＜Sample＞
・PCR productions
＜Protocol＞
SeeProtocol 4
3：DNA concentration measurement
＜Member＞
nito
＜Sample＞
・PCR products
＜Protocol＞
- Add 2µl of TE and 2µl of sample into the tube, and mix it gently.
- Fall in drops 1 on the measuring machine.
＜Result＞
| concentration | A320 | A260/A280 | A260/A230 | |
|---|---|---|---|---|
| 3HH | 39.0 | 0.500 | 1.814 | 0.161 |
| 3H | 23.5 | 0.051 | 1.858 | 0.091 |
| 3L | 39.5 | 0.400 | 1.829 | 0.272 |
| 3LL | 17.0 | 0.189 | 1.868 | 0.086 |
| 7HH | 28.5 | 0.070 | 1.894 | 0.099 |
| 7H | 27.0 | 0.045 | 1.829 | 0.046 |
| 7L | 38.0 | 0.057 | 1.900 | 0.175 |
| 7LL | 12.2 | 0.000 | 1.885 | 0.299 |
4：Electrophoresis
＜Member＞
Mariko, nito
＜Sample＞
・PCR products
＜Protocol＞
SeeProtocol 8
＜Result＞
