Weekends rock!!

Chris's first gel of the colony PCR. Lanes 1-3 are CpxP2 in AC Vector, Lanes 4-7 are CpxP2 in AK vector, Lanes 8-12 are CpxP1 in AC vector, Lanes 13017 are CpxP3 in AC vector and Lane 18 is CpxP3 in AK vector.

Chris

Today, I ran 1.0% gels of the enormous colony PCR that was done yesterday. The gels themselves can be seen to the right with labels. As well, I made overnight cultures of I0500-I13507 construction which will be plasmid prepped tomorrow. Finally, I helped Emily digest and phosphatase MalE so it could be inserted into the AK Biobrick vector.