Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/23

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Contents

2010/8/23 Monday (watachin)

Experiment:Direct PCR(K12-bcsA,K12-bcsC)

Member
Bambi75 and watachin

Materials

  • E.coli K12 colony
  • 10µM/l forward Primer 5µl(K12-bcsA,K12-bcsC)
  • 10µM/l reverse Primer 5µl(K12-bcsA,K12-bcsC)
  • 10×EX taq buffer 10µl
  • 2.5mM each dNTP mixture 10µl
  • EX taq polymerase 1µl
  • milli-Q 71µl


Procedure

follow the protocol


Experiment:Making media(LB broth + amp)

Member
Bambi75 and watachin

Material

  • LB media 200ml 
Distilled water 200ml
LB Broth 4g
  • ampicillin (50mg/ml) 200μl


Experiment:Restriction enzyme digestion

Member
Bambi75 and watachin

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