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Contents 1 Miniprep for pGFPrrnB with yneA 1.1 Aims 1.2 Materials and Protocol 1.3 Results 1.4 Discussion 2 Digestion of pGFPrrnB with insert yneA with hinDIII

Miniprep for pGFPrrnB with yneA

Aims

To produce more stocks of vector pGFPrrnB with insert yneA.

Materials and Protocol

Please refer to qiagen minipreps and nanodrop spectrophotometer for all 12 tubes of miniprep that we did.

Results

The results from the nanodrop:

Discussion

The results from the nanodrop showed that we have produced high concentration of vector pGFPrrnB with insert yneA. We will then proceed to digestion.

Digestion of pGFPrrnB with insert yneA with hinDIII