Team:Newcastle/27 July 2010

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Contents 1 Genomic DNA extraction experiment 1.1 Aims 1.2 Protocol 1.3 Discussion 1.4 Conclusion 2 Gel Electrophoresis 2.1 Protocol 2.2 Discussion 2.3 Conclusion

Genomic DNA extraction experiment

Aims

The aim of today's experiment is to extract genomic DNA from both B. subtilis strains 168 and 3610. The genes necessary for the swarming biobrick and rocF biobrick will then hopefully be obtained from the genomic DNA using...

Protocol

• Please refer to DNA extraction of Bacillus subtilis

Discussion

At the end of the DNA precipitation step, we did observe a small white pellet in all the eppendorf tubes.

Conclusion

The experiment was a success! The content and purity of the extracted DNA will be checked by using PCR on 28th July, 2010.

Gel Electrophoresis

Protocol

• Please refer to Gel Electrophoresis

Discussion

• Unfortunately no bands were observed on the gel.

Conclusion

The PCR did not work. It could be due to the DNA not being extracted or that we might have used the wrong primers. It could also be caused by the small size of the fragment that we amplified which ran off the gel because we ran the gel for too long.