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Team:Newcastle/2 July 2010
From 2010.igem.org
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Contents |
Urease Test
Aims
The aim of this experiment was to determine if Bacillus subtilis 168 is able to produce urease and degrade urea.
Materials
The experiment was performed on 01.07.10. For the materials used, please see 01.07.10 lab notebook.
Protocol
The experiment was performed on 01.07.10. For the experimental protocol, please see 01.07.10 lab notebook.
Results
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Figure 1: Urease test results
- Negative control - No color change (Orange color)
- Test 1 (Duplicate) - Pink-red color
- Test 2 (Duplicate) - Pink-red color
Discussion
B. subtilis 168 is able to produce urease, therefore, urea which is the substrate and can be found in the agar was degraded, which results in ammonia building. The ammonia makes the media alkaline and therefore the indicator phenol red will change from orange color to pink-red color.
Conclusion
The negative control did not turn pink-red color, therefore indicating that no contamination had occurred.
Competent E. coli Production
Aims
- To make a stock of competent E. coli (DH5alpha strain) ready for transformation.
Materials
- E. coli DH5alpha strain
Protocol
- Set up liquid culture of E. coli DH5alpha
Inference
- Grow up a stock of E.coli DH5alpha strain ready to be made competent
- Produce competent E. coli (DH5alpha strain)
   
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