Urease Test

Aims

The aim of this experiment was to determine if Bacillus subtilis 168 is able to produce urease and degrade urea.

Procedures

• Please refer to urease test

Inference

Christensen's Urea Agar was formulated to detect and differentiate urolytic and urea degrading microorganisms. The addition of gelatine peptone, dextrose and reduced content of buffer supports a luxuriant growth at an early stage. Urea is the substrate and can be degraded by certain organisms, which results in ammonia building. The ammonia makes the media alkaline and therefore the indicator phenol red will change from orange color to pink-red color.

• Negative control - No color change (Orange color)
• Test 1 (Duplicate) - Pink-red color
• Test 2 (Duplicate) - Pink-red color

For actual results, please see 02.07.10 lab notebook.

Competent E. coli Production

Aims

• To make a stock of competent E. coli (DH5α strain) ready for transformation.

Materials and Protocol

• Set up liquid culture consisting of E. coli DH5α.