20 July 2010

• Plating Asaia to get WT (once again)
• Making HEPES Buffer for the Competence of Asaia
• Diluted the culture to get a 25ml culture(1:20) according to Asaia competence protocol
• OD curve plotted, Doubling time calculated
• No single colonies from the re-growing trial --> replating properly on separate plates
• Preparation of LB medium mixed with Kan/Amp/Cm and GLY medium mixed with <kan
• Miniprep of Origin + Resistance
• Miniprep of Origin alone.
• Miniprep of base vector BBa_151020
• Concentration measurments of miniprep products
• Glycerol stock of BB plasmid and Base Vector made