Team:EPF Lausanne/Calendar/0721

From 2010.igem.org


21 July 2010

  • Measure OD of overnight culture of Asaia for competence --> value was too high
  • Diluted culture to 0.3 OD, left in 30 degree incubator for 2 hours, OD of 0.6 measured --> start with competence
  • Asaia competence
  • Digestion of BB's produced and of the Base vector
  • Agar plates with Kan/Tet/Cm/Amp made
  • Prepared Gel with the products of the digestion to check if the fragments are as expected
  • Prepare Tet stock (from powder)

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