21 July 2010

• Measure OD of overnight culture of Asaia for competence --> value was too high
• Diluted culture to 0.3 OD, left in 30 degree incubator for 2 hours, OD of 0.6 measured --> start with competence
• Asaia competence
• Digestion of BB's produced and of the Base vector
• Agar plates with Kan/Tet/Cm/Amp made
• Prepared Gel with the products of the digestion to check if the fragments are as expected
• Prepare Tet stock (from powder)