19 July 2010

• Results of the transformation checked: successful!!
• Amplification of colonies (goal to do a miniprep of the plasmids --ori-- and --ori-resistance--)
• Results of plating of asaia culture (where we hope to recover the WT) checked: Asaia cultures grew on Kan plates also! Checked colonies for fluorescance under the microscope: no fluorescance --> Very unexpected! Don't know reason yet.
• Re-grow the colonies. Multiple cycles: hope that we get WT.
• Preparation of Asaia cultures for Lemaitre experiments/WT/Competence...
• Preparation of SOC medium and GLY medium
• Autoclaving of media and flasks