14 July 2010

• Run a gel for the PCR of the previous day -> worked
• Purification of PCR’s product -> ok
• Preparation of the BBα_151020 (streak out)
• Competence Calculation of E.Coli DB3.1 (+pUC10, Amp) -> ok
• Look at Asaia with microscope -> we have cells (pictures)
• Growth Curve for Asaia (OD measurement each hour) -> both manually (ok) + with the plate reader
• Plated Asaia to recover some WT (streak out)
• Preparation of Asaia Culture (for Lemaître experiment) with Kan
• Protocol LateX template
• O/N culture of the E.coli DH5 transformed with the BB Antibiotics resistance (4x) -> miniprep
• Learning HTML for the wiki
• Text for the project presentation due to iGem
• Writing Protocols of basic procedures for the wiki page
• Culture of Asaia + Kan