15 July 2010

• MaxiPrep of Lemaître’s culture
• Purification of the BB resistances from the E.Coli cultures
• Protocol for cloning the Asaia Vector
• Enzyme digestion of the BB resistances and Asaia_ORI
• Cloning (Asaia_ORI + Vector/ Asaia_ORI + BB + Vector)
• Wiki text
• Preparation of LB agar + Cm
• Electrophoresis of resistance fragments (Works!!!) and extraction
• Purification of extracted DNA from agarose gel