Team:Calgary/16 July 2010
From 2010.igem.org
Notebook
|
||||||||||||||||||||||||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Friday July 16, 2010
Chris
Today, I made competent cells that are capable of surviving the ccdB gene (dbc-1). As well, I finished the moving of CpxP into Amp-Chlor and Amp-Kan plasmid backbones. We found on Monday that the plasmids never made it into plates and were left shaking at 200 rpm at 37 °C over the weekend. As well, I got into contact with Suncor Energy in hopes of finding some corporate sponsorship for our team.
Emily
Today I prepared a 20% w/v arabinose solution in order to induce our overnight cultures of a hypothetical I0500-B0034-E0040 with arabinose. I also worked on making glycerol stocks of the four plasmids that we have received from the Raivio lab in Edmonton in order to test our system with.
No notebook page exists for this date. Sorry!