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Team:Brown/Notebook/June24
From 2010.igem.org
Thursday, June 24 2010
Plated both immotile strains (DFB22, DFB 201) and three inducibly motile strains (pDB28, pDB101, pDB102) all in null media, amicillin + IPTG, and chloramphenicol + arabinose for a total of 6 x 3 = 18 plates and stabs (including stabs).
The expected results are:
| Strain | null plate | amp+IPTG | Chlor+arab |
|---|---|---|---|
| DFB 22 | immotile growth | no growth | no growth |
| DFB 201 | immotile growth | no growth | no growth |
| DFB 22 w/ pDB28 | immotile growth | motile growth | no growth |
| DFB 201 w/ pDB101 | immotile growth | no growth | motile growth |
| DFB 201 w/ pDB102 | immotile growth | motile growth | no growth |
MRS Plates
Notation for chlor is 2 stripes. Total – 500 mL
- 250 mL Null plates
- 250 mL Chloramphenicol plates at 15 µg/mL
Total = 3750 µg (3.75 mg)
Stock – 34 mg/mL
