Team:Brown/Notebook/June24

From 2010.igem.org

Thursday, June 24 2010

Plated both immotile strains (DFB22, DFB 201) and three inducibly motile strains (pDB28, pDB101, pDB102) all in null media, amicillin + IPTG, and chloramphenicol + arabinose for a total of 6 x 3 = 18 plates and stabs (including stabs).

The expected results are:

Strain null plate amp+IPTG Chlor+arab
DFB 22immotile growthno growthno growth
DFB 201immotile growthno growthno growth
DFB 22 w/ pDB28immotile growthmotile growthno growth
DFB 201 w/ pDB101immotile growthno growthmotile growth
DFB 201 w/ pDB102immotile growthmotile growthno growth

MRS Plates

Notation for chlor is 2 stripes. Total – 500 mL

  • 250 mL Null plates
  • 250 mL Chloramphenicol plates at 15 µg/mL

Total = 3750 µg (3.75 mg)

Stock – 34 mg/mL