Team:Caltech/Week 5

Monday 7/12

• Group meeting today!
• Sequencing results were really crappy (quality < 30% confidence), so began 2 5mL LB cultures of the light-lysis construct (picked from the original transformation plate).

Tuesday 7/13

• Sent off two new samples of the light-lysis construct for sequencing using the VF2 and VR primers. Results should be available tonight or tomorrow.
• Used remaining cells to inoculate new cultures of each and to extract DNA for a test digest:
  • Digested each (ideally identical) sample with EcoRI-HF and PstI, both for 1hr and for 10min.
  • The digest appears inconclusive, as a smear is visible from the top to about 3Kb, while little or no DNA is visible in the insert range (~1.5Kb, see below).

Gel image for the light-lysis test digest. (7/13)

• • Will run another gel tonight with other bricks to check efficacy of digests and will look forward to sequencing results soon.

Wednesday 7/14

Thursday 7/15

Friday 7/16

Weekend 7/17-18