Team:METU Turkey/Planning

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PLANNING

Cloning

- Design and ordering of DNA parts
- Preparation of competent cells
- Cloning
- Construction of Cell Sensor modules
- Validation
- Assembly of final construct

Cell Sensor Experiments

- Fermentor studies
- Flask experiments
- Solid culture experiments
- Myoglobin assay optimization
- Signal quantification with fluorescence spectroscopy
- Imaging with confocal laser scanning microscopy

CooA Expression and Purification

- Overexpression optimization
- Purification optimization

Characterization: Isothermal Titration Calorimetry (ITC)

- DNA component preparation
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities

Characterization: Electrophoretic Mobility Shift Assay (EMSA)

- Optimization studies
- Determination of Response Element - Transcription Factor Affinities

Characterization: Intrinsic Tryptophan Fluorescence (ITF)

- Optimization studies
- Determination of Response Element - Transcription Factor Affinities

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