Team:METU Turkey/Planning

 

PROJECT EXECUTION

Cloning
- Design and Synthesis of Response Elements
- Construction of Cell Sensor modules
- Preparation of competent cell
- Design and ordering of DNA parts
- Cloning
- Validation
- Assembly of final construct

Cell Sensor Experiments
- Fermentor studies
- Flask experiments
- Solid culture experiments
- Myoglobin assay optimization
- Signal quantification with fluorescence spectroscopy
- Imaging with confocal laser scanning microscopy

CooA Expression and Purification
- Overexpression optimization
- Purification optimization

Characterization: Isothermal Titration Calorimetry (ITC)
- DNA component preparation
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities

Characterization: Electrophoretic Mobility Shift Assay (EMSA)
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities

Characterization: Intrinsic Tryptophan Fluorescence (ITF)
- Optimization studies
- Determination of Response Element - Transcription Factor Affinities