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From 2010.igem.org

Double Click Bacteria

If there are two-time input in fixed time, bacteria is going to shine.

Overall Project

We’re inspired by double-click of computer’s mouse. It doesn’t react by first input but react by second input. So we designed bacterial DNA sequence which works like it. Furthermore, we built time-limit in genetic sequence between the first and the second input like a computer mouse. Bacteria which have the DNA sequence can distinguish between the first input and the second one. Once in a while, bacteria might receive input which wasn’t intended. But it was the first input so bacteria don’t react. Even if there was input not intended, bacteria don’t react unless the second input is entered. Moreover, when the limit-time which controlled by the genetic system is passed, the system is going to return to the initial state. So we expect this DNA sequence will work as a safety device.

Requirement of Double Click

1. If there is a click only one-time, nothing happens.
2. Time of clicking button doesn't matter. It just depends on the number of click. One or two.
3. After a lot of time pass from the 1st click, it will return to the initial state.
4. Click two-time in specific limited time(We call this double click.), something occur.