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Design and ordering of DNA parts
- Ordering of parts from GENEART: We have ordered 11 gene sequences from GENEART. We have synthesized pCooM, pCooF promoter and its 4 variants, CooA protein and 4 of its variants. Those genes are proven to be successful in cloning.
- Design of parts: We have designed two different vectors , one containing pTriEx vector containing CooA and one containing our final construct. However, we couldn’t observe fluorescence with this system because of high expression capability of pTriEx. Because of this factor, we have designed another biobrick containing pLacI-RBS-RFP. We have cloned this with pCooM-RBS-RFP-TT and pSB1C3. We didn’t have enough time for cloning of the final vector with this biobrick.
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