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Team:Tsinghua/Notebook/9 October 2010
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Module I, Group 1A
Colony pcr to screen the clones in which landing pad has successfully integrated into genome, using up primers from the plasmid and down primers from the genome.
Module I, Group 2c
After purification, ligation reaction took place in a mixed system as following， respectively.
eGFP 3ul PSB1C13 2ul T4 ligase(NEB) 1ul T4 ligation Buffer 2ul H2O 12ul
mCherry 2ul PSB1C13 2ul T4 ligase(NEB) 1ul T4 ligation Buffer 2ul H2O 13ul
Kan 3ul PSB1C13 2ul T4 ligase(NEB) 1ul T4 ligation Buffer 2ul H2O 12ul
Incubate at 16 centidegree overnight.
