User contributions

From 2010.igem.org

• 15:59, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/18 October 2010 ‎ (New page: ==Group 1A== Colony PCR to screen for clones in which KM gene has successfully taken the part of tet in genome, using up primers from donor plasmid and down primers from genome.) (top)
• 15:57, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/17 October 2010 ‎ (New page: ==Group 1A== Dilute and spread the bacteria to km plates. Incubate overnight.) (top)
• 15:55, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/16 October 2010 ‎ (New page: ==Group 1A== Select a few clones to amplify in LB media. When OD600 reaches 0.6, transfer 1ml liquid to new media. Add IPTG and L-arabinose when OD 600 reaches 0.6. After 1 hour, add km to...) (top)
• 15:49, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/15 October 2010 ‎ (New page: ==Group 1A== Select a few clones to amplify. When OD 600 reaches 0.6, make the strain to competent for electrotransformation. Transform donnor plasmids to the competents. Spread to plates ...) (top)
• 15:46, 27 October 2010 (diff | hist) Team:Tsinghua/Notebook/14 October 2010 ‎ (→Group 1A) (top)
• 15:44, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/14 October 2010 ‎ (New page: ==Group 1A== Select a few clones with tet in genome and incuble to 0.6 OD600. Make competent of this strain for electrotransformation.)
• 15:35, 27 October 2010 (diff | hist) Team:Tsinghua/Notebook/9 October 2010 ‎ (→Module I, Group 1A) (top)
• 15:14, 27 October 2010 (diff | hist) Team:Tsinghua/Notebook/9 October 2010 ‎ (→Module I, Group 2c)
• 15:03, 27 October 2010 (diff | hist) Team:Tsinghua/Notebook/6 October 2010 ‎ (top)
• 15:01, 27 October 2010 (diff | hist) Team:Tsinghua/Notebook/8 October 2010 ‎ (→Module I, Group 2c) (top)
• 14:30, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/25 July 2010 ‎ (New page: Group 1B ---- NO positive clones after 24 hours.) (top)
• 14:28, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/24 July 2010 ‎ (New page: Group 1B ---- Transform the plasmid ptv into DH5α. Spread to tetracyclin plates.) (top)
• 12:56, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/23 July 2010 ‎ (New page: Group 1B ---- Ligate pt and v in T4 ligation system.) (top)
• 12:43, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/22 July 2010 ‎ (New page: Group 1B ---- Overlap PCR to produce pt again. There were both expected bands and unexpected bands after running a gel. Use the product as templates and pcr again. Extract the desired pro...) (top)
• 11:04, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/21 July 2010 ‎ (New page: Group 1B ---- Overlap PCR with promoters (p) and tetracyclin gene (t) fragments. PCR for 7 cycles without primers, then add up primers of promoters and down primers of tetracyclin gennes...) (top)
• 10:49, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/20 July 2010 ‎ (New page: GROUP 1B ---- PCR promoters (p), tetracyclin gene (t) and vector (v) in gradient temperature to test the best condition.) (top)
• 10:47, 27 October 2010 (diff | hist) Team:Tsinghua/Notebook/1 October 2010 ‎ (top)
• 10:47, 27 October 2010 (diff | hist) Team:Tsinghua/Notebook/1 October 2010 ‎
• 10:41, 27 October 2010 (diff | hist) Team:Tsinghua/Notebook/6 October 2010 ‎
• 10:21, 27 October 2010 (diff | hist) Team:Tsinghua/team/team ‎ (→The Students)
• 10:16, 27 October 2010 (diff | hist) Team:Tsinghua/team/team ‎ (→The Students)
• 04:03, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/23 September 2010 ‎ (New page: Group 1B ---- No positive clones. We decided to change our project.) (top)
• 03:59, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/22 September 2010 ‎ (New page: Group 1B ---- Transform the ligation system into DH5α. Spread on tetracyclin plates.) (top)
• 03:57, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/7 October 2010 ‎ (New page: Group 1A ---- Amplification of plasmid ptv in LB media with 0.05% tetracyclin.) (top)
• 03:54, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/21 September 2010 ‎ (New page: '''Group 1B''' ---- Gel extraction of digested fragments. Ligate the fragments in T4 ligation system.)
• 03:51, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/20 September 2010 ‎ (New page: '''GROUP 1B'''---- Overlap pcr to ligate p and t. Gel extraction. Enzyme digestion with SalI and XbaI.)
• 03:50, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/19 September 2010 ‎ (New page: GROUP 1B ---- Overlap pcr to ligate p and t together. NO positive results.)
• 03:42, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/18 September 2010 ‎ (New page: '''GROUP 1B''' ---- PCR the fragments of promoters (p), tetracyclin gene (t) from plasmid pTKS/CS. PCR the fragment of vector (v) with ori and attB sequence from plasmid pUK.) (top)
• 03:39, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/6 October 2010 ‎ (New page: GROUP 1A ---- Repeat construct the plasmid ptv with promoter (p), tetracyclin(t), and vector (v).)
• 03:31, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/5 October 2010 ‎ (New page: GROUP 1A ---- No clones found after 16h.) (top)
• 03:07, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/4 October 2010 ‎ (New page: '''GROUP 1A''' ---- Transform 10μl ligation system to pAH69 competent by electrotransformation. Spread to plates with tetracyclin. Incubate at 30℃.) (top)
• 02:37, 27 October 2010 (diff | hist) Nm Team:Tsinghua/Notebook/30 September 2010 ‎ (New page: '''Group 1A''' ---- Amplification of the strain with plasmid pAH69. Prepare competent of this strain for electrotransformation.)
• 02:27, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/3 October 2010 ‎ (New page: '''GROUP 1A''' Ligate the two fragments in T4 ligation system.) (top)
• 02:25, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/2 October 2010 ‎ (New page: '''GROUP 1A''' Digest the fragments with SalI and XbaI overnight. Gel extraction.) (top)
• 02:19, 27 October 2010 (diff | hist) N Team:Tsinghua/Notebook/1 October 2010 ‎ (New page: '''Group 1a''' PCR the fragments of vector with ori and attB sequence and landing pad including promoter and tetracyclin gene. Gel extraction.)
• 02:04, 27 October 2010 (diff | hist) Team:Tsinghua/project/outline/m1 ‎ (→Landing Pad Construction and Insertion)