Team:Queens-Canada/29 July 2010

Gel Electrophoresis

Hao

pSrb-6, pCeh-12, pOdr-10, NpHR, ChR2, pOsm-10, pStr-1, eCFP, pHsp-3, pFlp-1, pSB1A3, pStr-220

Used 0.7 g agarose in 100 mL TBE. Gel run for 60 mins at 100 V.

Gel Electrophoresis

Thai

eGFP, pMec-7, pRab-7, pSip-1

Used 0.3 g agarose in 45 mL TBE. Gel run for 45 mins at 100 V.

Gel Extraction

Yuli

eGFP

Used 30 µL elution buffer.

Liquid Culture

Hao

pSB1T3, pSB1C3, pSB1K3

Liquid Culture

Yuli

pStr-1, pSB1A3, pSip-1::1A3, pRab-7::1A3, pMec-7::1A3, unc-54 3'UTR::1A3