Team:Queens-Canada/29 July 2010

From 2010.igem.org

July 29, 2010

Gel Electrophoresis

Hao

pSrb-6, pCeh-12, pOdr-10, NpHR, ChR2, pOsm-10, pStr-1, eCFP, pHsp-3, pFlp-1, pSB1A3, pStr-220

Used 0.7 g agarose in 100 mL TBE. Gel run for 60 mins at 100 V.

Gel Electrophoresis

Thai

eGFP, pMec-7, pRab-7, pSip-1

Used 0.3 g agarose in 45 mL TBE. Gel run for 45 mins at 100 V.

Gel Extraction

Yuli

eGFP

Used 30 ┬ÁL elution buffer.

Liquid Culture

Hao

pSB1T3, pSB1C3, pSB1K3

Liquid Culture

Yuli

pStr-1, pSB1A3, pSip-1::1A3, pRab-7::1A3, pMec-7::1A3, unc-54 3'UTR::1A3