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Team:Queens-Canada/29 July 2010
From 2010.igem.org
July 29, 2010
Gel Electrophoresis
Hao
pSrb-6, pCeh-12, pOdr-10, NpHR, ChR2, pOsm-10, pStr-1, eCFP, pHsp-3, pFlp-1, pSB1A3, pStr-220
Used 0.7 g agarose in 100 mL TBE. Gel run for 60 mins at 100 V.
Gel Electrophoresis
Thai
eGFP, pMec-7, pRab-7, pSip-1
Used 0.3 g agarose in 45 mL TBE. Gel run for 45 mins at 100 V.
Gel Extraction
Yuli
eGFP
Used 30 µL elution buffer.
Liquid Culture
Hao
pSB1T3, pSB1C3, pSB1K3
Liquid Culture
Yuli
pStr-1, pSB1A3, pSip-1::1A3, pRab-7::1A3, pMec-7::1A3, unc-54 3'UTR::1A3
