Team:Calgary/19 September 2010

From 2010.igem.org

Revision as of 01:02, 27 October 2010 by Cktang (Talk | contribs)

Sunday September 19, 2010

Weekends? Who needs those?

The restriction digests of the CpxP and ibpAB-fsxA promoters with XbaI and PstI to verify and run on a gel

Chris

Today, I did plasmid preparations of the CpxP and ibpAB-fsxA fusion promoter overnight cultures that were set up yesterday. The concentrations are available on the tubes. Some of the promoters were set up in AK and AC in order to facilitate future construction. Then, I set up restriction digests using XbaI and PstI to be run on a gel as the final step in verification. I also set up a DNA PCR of the CpxP and ibpAB which will be run on a gel tomorrow.