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From 2010.igem.org

BBa_K381001

BBa_K381001 is our key biobrick for this project; encoding expression of GFP in response to Nitrates or Nitrites. During the project we both made and well characterised this part, details of which can be found in our Experiment section. Information on its sequence and how to get hold of it can be found at the [http://partsregistry.org/Part:BBa_K381001 BBa_K381001] page on the parts registry website.

Part Design

BBa_K381001 is a composite part of two pre-existing biobricks taken from the parts registry; a Nitrate sensitive promoter and a GFP generator, along with strong RBS and transcription terminators.

The promoter is BBa_K216005, originally submitted by Edinburgh in 2009, whilst the GFP generator, along with strong RBS and transcription terminators is BBa_E0840, provided in well 12O, kitplate 1 of the 2010 distribution.

Performance

Performance of the promoter specifically was assessed using a Miller Assay. Whilst performance of the biobrick as a whole was characterised with an assay measuring change in GFP expression with changes in Potassium Nitrate levels.