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Team:Washington/Gram Positive/Test/MutantDataTable
From 2010.igem.org
Mutant Data Table
| Mutant Name | Features and Mutations | Transpeptidation per DCP | Hydrolysis per DCP |
| CapD | No Signal Sequence, Triple band on protein agarose gel | 0.491 | 0.713 |
| CapD_CP | Foldit created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 1.000 | 1.000 |
| CapD_CPNFDelta | Foldit created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel, Removed "floppy" section to compact protein | 1.035 | 0.878 |
| F24A | Point mutation at residue 24 on CapDCP from Phenylalanine to Alanine, Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.021 | 0.061 |
| F24H | Point mutation at residue 24 on CapDCP from Phenylalanine to Histidine, Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.108 | 1.205 |
| F24H, L40R, T59D_M61S | Point mutation at residues 24, 40, 59, and 61 on Foldit design from Phenylalanine to Histidine, Leucine to Arginine, Threonine to Asparagine, and Methionine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.001 | 0.006 |
| F24H, L40R, T59N_M61S | Point mutation at residues 24, 40, 59, and 61 on Foldit design from Phenylalanine to Histidine, Leucine to Arginine, Threonine to Aspartic Acid, and Methionine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.058 | 1.050 |
| F24H, L40R, T59S_M61S | Point mutation at residues 24, 40, 59, and 61 on CapDCP from Phenylalanine to Histidine, Leucine to Arginine, Threonine to Serine, and Methionine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.000 | 0.007 |
| F24H, R356K | Point mutation at residues 24 and 356 on CapDCP from Phenylalanine to Histidine and Arginine to Lysine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.129 | 0.257 |
| F24H, T59R | Point mutation at residues 24 and 59 on CapDCPfrom Phenylalanine to Histidine and Threonine to Arginine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.011 | 0.005 |
| F24H,T59N | Point mutation at residues 24 and 59 on CapDCP from Phenylalanine to Histidine and Threonine to Asparagine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.007 | 0.029 |
| F24W | Point mutation at residue 24 on CapDCP from Phenylalanine to Tryptophan. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.006 | 0.044 |
| F24Y | Point mutation at residue 24 on CapDCP from Phenylalanine to Tyrosine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.593 | 0.887 |
| F24Y, L40R | Point mutation at residues 24 and 40 on CapDCP from Phenylalanine to Tyrosine and Leucine to Arginine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.009 | -0.013 |
| F24Y, L40R, T59N | Point mutation at residues 24, 40, 59 on CapDCP from Phenylalanine to Tyrosine, Leucine to Arginine, and Threonine to Asparagine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.003 | 0.157 |
| F24Y,R305K | Point mutation at residues 24 and 305 on CapDCP from Phenylalanine to Tyrosine and Arginine to Lysine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.002 | 0.233 |
| F24Y,T59N | Point mutation at residues 24 and 59 on CapDCP from Phenylalanine to Tyrosine and Threonine to Asparagine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.008 | 0.165 |
| F24Y,T59N,R305K | Point mutation at residues 24, 59, and 356 on CapDCP from Phenylalanine to Tyrosine, Threonine to Asparagine, and Arginine to Lysine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.002 | 0.127 |
| F60W | Point mutation at residue 60 on CapDCP from Phenylalanine to Tryptophan. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.239 | 0.489 |
| G79A | Point mutation at residue 79 on CapDCP from Glycine to Alanine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.040 | -0.511 |
| I83T | Point mutation at residue 83 on CapDCP from Isoleucine to Threonine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.009 | 0.006 |
| I83V | Point mutation at residue 83 on CapDCP from Isoleucine to Valine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.003 | 0.043 |
| L40A | Point mutation at residue 40 on CapDCP from Leucine to Alanine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.150 | 0.413 |
| L40R | Point mutation at residue 40 on CapDCP from Leucine to Arginine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 1.157 | 1.527 |
| L40S | Point mutation at residue 40 on CapDCP from Leucine to Serine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.166 | 0.577 |
| L40W | Point mutation at residue 40 on CapDCP from Leucine to Tryptophan. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.825 | 1.215 |
| M61H | Point mutation at residue 61 on CapDCP from Methionine to Histidine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | -0.003 | 0.002 |
| M61N | Point mutation at residue 61 on CapDCP from Methionine to Asparagine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.011 | 0.002 |
| M61S | Point mutation at residue 61 on CapDCP from Methionine to Serine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.262 | 0.485 |
| N23K | Point mutation at residue 23 on CapDCP from Asparagine to Lysine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.818 | 0.839 |
| N23Q | Point mutation at residue 23 on CapDCP from Asparagine to Glutamine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.165 | 0.646 |
| S143K | Point mutation at residue 143 on CapDCP from Serine to Lysine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.574 | 0.426 |
| S22I, T59A | Point mutation at residues 22 and 59 on CapDCP from Serine to Isoleucine and Threonine to Alanine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.030 | -0.057 |
| S22Q, T59Q | Point mutation at residues 22 and 59 on CapDCP from Serine to Glutamine and Threonine to Glutamine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | -0.012 | 0.085 |
| S57H, M61H | Point mutation at residues 57 and 61 on CapDCP from Serine to Histidine and Methionine to Histidine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | -0.004 | -0.007 |
| T18L, T59Q, M61N, F452W | Point mutation at residues 18, 59, 61, and 452 on CapDCP from Threonine to Leucine, Threonine to Glutamine, Methionine to Asparagine, and Phenylalanine to Tryptophan, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.020 | -0.028 |
| T18S_T20S | Point mutation at residues 18 and 20 on CapDCP from Threonine to Serine and Threonine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.617 | 1.186 |
| T20A | Point mutation at residue 20 on CapDCP from Threonine to Alanine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.011 | 0.021 |
| T20C | Point mutation at residue 20 on CapDCP from Threonine to Cystine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.090 | 0.142 |
| T20S | Point mutation at residue 20 on CapDCP from Threonine to Serine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.639 | 1.443 |
| T20S, L40D | Point mutation at residues 20 and 40 on CapDCP from Threonine to Serine and Leucine to Asparagine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.853 | 0.001 |
| T20S, L40E | Point mutation at residues 20 and 40 on CapDCP from Threonine to Serine and Leucine to Glutamic Acid, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.163 | 0.637 |
| T20S, L40F | Point mutation at residues 20 and 40 on CapDCP from Threonine to Serine and Leucine to Phenylalanine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.246 | 0.335 |
| T20S, L40H | Point mutation at residues 20 and 40 on CapDCP from Threonine to Serine and Leucine to Histidine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.001 | 0.012 |
| T20S, L40K | Point mutation at residues 20 and 40 on CapDCP from Threonine to Serine and Leucine to Lysine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.000 | 0.007 |
| T20S, L40Q | Point mutation at residues 20 and 40 on CapDCP from Threonine to Serine and Leucine to Glutamine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.126 | 0.478 |
| T20S, L40R | Point mutation at residues 20 and 40 on CapDCP from Threonine to Serine and Leucine to Arginine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.000 | 0.009 |
| T20S, L40R, F60E | Point mutation at residues 20, 40, and 60 on CapDCP from Threonine to Serine, Leucine to Arginine, and Phenylalanine to Glutamic Acid, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.048 | 0.028 |
| T20S, L40R, F60Q | Point mutation at residues 20, 40, and 60 on CapDCP from Threonine to Serine, Leucine to Arginine, and Phenylalanine to Glutamine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.002 | 0.007 |
| T20S, L40R, N81E | Point mutation at residues 20, 40, and 60 on CapDCP from Threonine to Serine, Leucine to Arginine, and Phenylalanine to Glutamine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.001 | -0.004 |
| T20S, L40R, N81Q | Point mutation at residues 20, 40, and 81 on CapDCP from Threonine to Serine, Leucine to Arginine, and Asparagine to Glutamine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.422 | 0.321 |
| T20S, L40R, T59K | Point mutation at residues 20, 40, and 59 on CapDCP from Threonine to Serine, Leucine to Arginine, and Threonine to Lysine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.048 | 0.033 |
| T20S, L40R, T59K_M61S | Point mutation at residues 20, 40, 59, and 61 on CapDCP from Threonine to Serine, Leucine to Arginine, Threonine to Lysine, and Methionine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.010 | 0.032 |
| T20S, T59Q, M61T | Point mutation at residues 20, 59, and 61 on CapDCP from Threonine to Serine, Threonine to Glutamine, and Methionine to Threonine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.007 | -0.069 |
| T20S,M61A | Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Alanine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.005 | 0.045 |
| T20S,M61C | Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Cystine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.102 | 0.207 |
| T20S,M61D | Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Aspartic Acid, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.004 | 0.003 |
| T20S,M61G | Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Glycine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.004 | 0.029 |
| T20S,M61L | Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Leucine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.004 | 0.029 |
| T20S,M61N | Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Asparagine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.003 | 0.002 |
| T20S,M61S | Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.007 | 0.053 |
| T20S,M61T | Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Threonine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.038 | 0.363 |
| T20S,M61V | Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Valine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.004 | 0.039 |
| T20S,T59N | Point mutation at residues 20 and 59 on CapDCP from Threonine to Serine and Threonine to Asparagine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.019 | 0.125 |
| T20S,T59S_M61S | Point mutation at residues 20, 59, and 61 on CapDCP from Threonine to Serine, Threonine to Serine, and Methionine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.300 | 0.719 |
| T20S_F24H | Point mutation at residues 20 and 24 on CapDCP from Threonine to Serine and Phenylalanine to Histidine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.008 | 0.097 |
| T20S_F24Y | Point mutation at residues 20 and 24 on CapDCP from Threonine to Serine and Phenylalanine to Tyrosine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.040 | 0.201 |
| T20S_F24Y,T59N | Point mutation at residues 20, 24, and 59 on CapDCP from Threonine to Serine, Phenylalanine to Tyrosine, and Threonine to Asparagine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.013 | 0.048 |
| T2A | Point mutation at residue 2 on CapDCP from Threonine to Alanine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.007 | -0.020 |
| T2C | Point mutation at residue 2 on CapDCP from Threonine to Cystine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.001 | -0.005 |
| T2S | Point mutation at residue 2 on CapDCP from Threonine to Serine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.024 | 0.325 |
| T2S,T18S_T20S | Point mutation at residues 2, 18, and 20 on CapDCP from Threonine to Serine, Threonine to Serine, and Threonine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.144 | 0.250 |
| T2S,T18S_T20S,T59S_M61S | Point mutation at residues 2, 18, 20, 59, and 61 on CapDCP from Threonine to Serine, Threonine to Serine, Threonine to Serine, Threonine to Serine and Threonine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.003 | 0.022 |
| T2S,T20S | Point mutation at residues 2 and 20 on CapDCP from Threonine to Serine and Threonine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.106 | 0.089 |
| T2V | Point mutation at residue 2 on CapDCP from Threonine to Valine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.000 | 0.000 |
| T59H_M61A | Point mutation at residues 59 and 61 on CapDCP from Threonine to Histidine and Methionine to Alanine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.000 | 0.000 |
| T59H_M61T | Point mutation at residues 59 and 61 on CapDCP from Threonine to Histidine and Methionine to Threonine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.000 | 0.000 |
| T59M | Point mutation at residue 59 on CapDCP from Threonine to Methionine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.008 | 0.008 |
| T59N | Point mutation at residue 59 on CapDCP from Threonine to Asparagine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.232 | 0.868 |
| T59Q | Point mutation at residue 59 on CapDCP from Threonine to Glutamine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.000 | 0.000 |
| T59Q, M61Q, F452W | Point mutation at residues 59, 61, and 452 on CapDCP from Threonine to Glutamine, Threonine to Glutamine, and Phenylalanine to Tryptophan, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | -0.014 | 0.037 |
| T59S | Point mutation at residue 59 on CapDCP from Threonine to Serine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 3.525 | 1.047 |
| T59S, M61H | Point mutation at residues 59 and 61 on CapDCP from Threonine to Serine and Methionine to Histidine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel | 0.013 | 0.051 |
